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LORA Summary

Report created with LORA (v1.0.1)

LORA Report →

Overview

LORA (Long Read Assembly) is a Snakemake pipeline for de novo assembly of long-read sequencing data (PacBio, Oxford Nanopore). It covers the full workflow from raw reads to polished, annotated assemblies, and integrates quality assessment tools including QUAST, BUSCO, CheckM, Bandage, and Sequana coverage analysis.

Global reports

Per-sample reports

The table below lists each sample with the number of assembled contigs and links to the QUAST assembly quality report and Icarus contig browser. A link to the Sequana coverage report is included when coverage analysis was performed.

Sample Contigs Links
hifi 2 FASTA QUAST Icarus Coverage
hifi2 2 FASTA QUAST Icarus Coverage
hifi3 2 FASTA QUAST Icarus Coverage
hifi4 2 FASTA QUAST Icarus Coverage
hifi5 2 FASTA QUAST Icarus Coverage

Pipeline graph

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snakemake_dag 0 lora 1 create_svg 1->0 3 multiqc 3->0 4 quast 4->3 5 fastp 5->4 7 flye 5->7 10 minimap2 5->10 6 seqkit_sort 6->4 8 sequana_coverage 6->8 6->10 11 busco 6->11 12 prokka 6->12 14 seqkit_head 6->14 15 checkm 6->15 7->6 17 bandage 7->17 8->3 9 bam2bed 9->8 10->9 11->3 12->3 13 blast 13->3 14->13 15->3 16 checkm_marker 16->15 17->3

Software versions

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flye 2.9.3-b1797
multiqc 1.27
quast 5.2.0
samtools 1.16.1
seqkit v2.10.1
seqtk 1.3.0
blast 2.14.1+
busco 6.0.0
prokka 1.14.6
minimap2 2.30-r1287
sequana_coverage 0.20.0